Microbiology

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Final Year Project in Microbiology

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    ANTIBIOTICS PROFILING OF BACTERIA ISOLATED FROM CATFISH HARVESTED FROM AFELELE RIVER
    (2022) ADERINKOLA, Adenike Tayibat
    Fishes are cheap sources of protein and are commonly reared in aquaculture systems such as rivers in Nigeria. Bacterial infested fishes have been a serious public health concern. This study was aimed at isolating and identifying bacteria associated with the internal organs (gills, intestine, guts) of Clarias gariepinus (African Catfish) in Afelele river in Offa, kwara state, Nigeria. In addition, the bacterial load of the organs and antibiotic susceptibility profile of the isolates to conventional antibiotics were determined. An African catfish was sampled from the river. Bacteria isolated on Mac-Conkey agar and Nutrient agar plates were identified based on morphological and biochemical characteristics. Susceptibility of the isolates to antibiotics was carried out using the Kirby-Bauer disc diffusion method. Isolates from the fish organs include species of Vibrio, Aeromonas, Pseudomonas, Klebsiella, Staphylococcus, Serratia, Proteus, Bacillus, Streptococcus and Micrococcus. Results also revealed that there was significant difference in the bacterial load recovered from the fish organs (gut, gill and intestine). Serratia sp. was the most abundant gram positive bacterium in the organs of the fish sample, while Pseudomonas sp., a gram negative organism, appeared in the intestine and gut. Percentage susceptibility of the bacteria to antibiotics was highest with Ciprofloxacin and Gentamycin (85%) and least with Ampicillin and Vancomycin (75.0%). The most susceptible isolates were species of Bacillus and Klebsiella while Staphylococcus specie was the most resistant bacterium. This study has shown that high bacteria load are found in the internal organs of the fish and a good number of the bacteria are resistant to some of the antibiotics tested. Therefore, there is a need for adherence to proper sanitary measures to avoid bacterial contamination of fish.