Microbiology

Permanent URI for this collectionhttps://dspace.summituniversity.edu.ng/handle/123456789/24

Final Year Project in Microbiology

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    BIOPROCESS OPTIMIZATION OF MICROBIAL OIL YIELD FROM Aspergillus niger USING MACHINE LEARNING MODELS AND CORNCOB AS CARBON SOURCE
    (2024) MUHEEB, Wasilat Opeyemi
    Microbial oils present a sustainable alternative to traditional fats, and Aspergillus niger emerges as an ideal candidate due to its exceptional production capacity and genetic engineering. By utilizing corncob, a readily available agricultural residue, this research not only offers a cost-effective solution but also aligns with the principles of environmental sustainability. The optimization of bioprocess plays an essential involvement in maximizing oil yield, and genetic algorithms provide an efficient approach to identify the optimum conditions. This study, Consequently addresses global challenges such as the scarcity of fossil fuels and the necessity for environmental sustainability by Supporting the transition towards a bio-based economy and enhancing our understanding of bioprocess engineering. Samples were collected from two different dump sites, PDA was used to isolate fungal using serial dilution and pour plate method, screening for lipolytic activities using Congo red Agar media, Yeast Extract Peptone Dextrose broth was used for fermentation with Corncob and Glucose. Aspergillus niger was identified with the aid of microscope, the utilization of microbial lipid was obtained from the dried biomass based on Bligh and Dyer extraction methods and decision trees was used for prediction. Five pure isolates of different strains of fungal were obtained on PDA plates after incubation at 20-25°С. Two fungal isolates show a positive clear zone of precipitation around the pure fungal isolate, of which one organism was studied. Using the results for the fermentation, it was observed that Day 11 has the highest growth rate of 16.02g for the wet Oleaginous fungi while 2.88g for the dried and for these results. The study has shown that Oleaginous fungi can produce lipids that are beneficial for biodiesel production through the utilization of Corncob.