Microbiology
Permanent URI for this collectionhttps://dspace.summituniversity.edu.ng/handle/123456789/24
Final Year Project in Microbiology
Browse
1 results
Search Results
Item PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND ANTIBACTERIAL ACTIVITIES OF LEAF EXTRACT OF CHERRY MANGO AGAINST SELECTED CLINICAL ISOLATES(2024) ABDULAZEEZ AISHAT OPEYEMIThe project "Preliminary Phytochemical Investigation and Antibacterial Activities of Leaf Extract of Cherry Mango against Selected Clinical Isolates" explores the medicinal potential of cherry mango leaves. It aims to identify bioactive compounds in the leaf extract and evaluate their antibacterial properties against clinical bacterial isolates. The study involves extracting leaves using various solvents, screening for phytochemicals, and testing antibacterial activity against Gram-positive and Gram-negative bacteria. Preliminary findings indicate the presence of several therapeutic phytochemicals and significant antibacterial effects, suggesting the extract's potential as a natural antibacterial agent. The research supports the traditional use of cherry mango leaves in herbal medicine and contributes to developing new plant-based antimicrobials. Further studies are recommended to isolate active compounds and assess their clinical efficacy and safety. The study investigates the phytochemical composition and antibacterial properties of cherry mango leaf extract against specific clinical bacteria strains. This research aims to identify potential bioactive compounds present in the leaf extract and evaluate their effectiveness in inhibiting bacterial growth, providing valuable insights into the medicinal properties of cherry mango leaves. Standard methods were employed to screen for the phytochemicals. Agar well diffusion method was used to determine the antimicrobial effects of methanolic extracts of Mangifera Indica leaves against four different clinical isolates namely: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi which S. aureus has the lowest zone of inhibition (22mm) while S. typhi has no zone of inhibition in leave while in bark, P. aeruginosa has the highest (22mm) and S. typhi has the highest zone of inhibition (25mm) and were compared with standard antibiotics which also high zones of inhibition. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract was done using broth dilution and the activity of different extracts will be compared. Aqueous extract will be demonstrated at a higher activity than the ethanolic extracts Staphylococcus aureus will show highest sensitivity to the aqueous extract to the least sensitivity will be observed in the Klebsiella pneumoniae with each in the two extract Mangifera Indica will exhibit a significant antimicrobial activity compare to gentamicin will be used as control.