ISOLATION, SCREENING AND PRODUCTION OF MICROBIAL OIL YIELD FROM OLEAGINOUS MICROORGANISMS

Abstract

This study intends to isolate oleaginous microorganisms (fungi) from oil-rich soil and optimize bioprocesses to increase microbial oil yield. First, prospective oleaginous fungi in oil-rich soil must be isolated. The objective is to identify lipid-producing bacteria that can develop quickly in their natural environment. A screening procedure is used to determine which fungi produce the greatest lipids after they have been isolated. Usually, to achieve this, isolated fungi are cultured in environments that encourage lipid buildup. There are numerous screening methods that can be used. The ideal Oleaginous fungi (Aspergillus niger)growing conditions must be achieved in order to maximize lipid synthesis. These microbes grow and accumulate lipids in response to factors like temperature, pH, nutrient concentrations, and the availability of carbon sources. The process of removing the lipids from the cells comes next when the fungi have gathered enough lipids. It is possible to refine the recovered lipids further to produce pure oils suitable for use in biofuels or other high-value goods. The results made it clear that low-cost carbon sources must be used to grow these microorganisms and that the bioprocess's performance needs to be improved in terms of both yield and productivity. Oleaginous fungi can accumulate more lipids than 20% of their dry biomass. A variety of species of yeasts and filamentous fungi are categorized as oleaginous because they have the capacity to synthesize and store considerable amounts of TAG within their cells—up to 70% of the biomass weight. Single cell oil (SCO) production methods using heterotrophic oleaginous microorganisms have received a lot of attention recently. Due to their outstanding efficiency at accumulating intracellular TAG, oleaginous fungi, particularly yeasts, are projected to be utilized by the biofuel industry. The absence of readily available , reasonably priced feed stocks is the main issue facing the biodiesel industry. A successful method to boost microbial lipid productivity and lower the cost of microbial biofuel production is to regulate environmental conditions and optimize environmental parameters to improve the synthesis of microbial lipid.