ANTIBIOGRAM OF ISOLATED BACTERIA FROM SELECTED SACHET WATER IN OFFA, KWARA STATE AND DATASET COLLECTION FOR PREDICTIVE MODEL

Abstract

In Nigeria, sachet water is the only reliable source of drinking water. Aiming to evaluate the prevalence and antibiogram of bacteria isolated from specific brands of sachet water in Offa, Kwara State, this study attempts to evaluate the health concerns posed by diseases contracted from eating contaminated water internationally. Ten different brands of sachet water available in Offa, Kwara state, were examined using established techniques to ascertain the pH and bacteriological purity. Two bacterial isolates were characterized by sequencing the 16S rDNA using the universal primers 27F and 1492R to amplify the 16S target region. All the samples were clear and odourless with the exception of Sc4. Antibiotic susceptibility testing was performed using the disc diffusion method. Some bacterial isolates cannot be taxonomically identified from phenotypic characteristics. The water samples' pH values ranged from 1.7 to 7.0. Out of the ten water samples, bacterial growths were found in every single one. The range of the total heterotrophic count in cfu/ml was 2.0 to 5.8. Most of the water samples contained coliforms, with a value ranging from 1.1 to 7.0x103 cfu/ml. Staphylococcus aureus, Pseudomonas sp, Alcaligenes feacalis, Bacillus cereus, Proteus vulgaris, and Escherichia coli were among the isolates that were detected. Most isolates were shown to be extremely susceptible to ciprofloxacin, ofloxacin, and gentamycin while being resistant to ceftriazone sulbactam, cefotaxime, and amoxicillin clavulanate. Except for Escherichia coli, the majority of the isolates were susceptible to Gentamycin (GN), and all but one of them were resistant to Ceftriaxone sulbactam. Since none of the sachet water samples examined in this study met WHO standards for drinking water, routine monitoring of sachet water makers ought to be mandated.