ANTIOXIDANT AND ANTIDYSLIPIDEMIC EFFECT OF ETHANOLIC EXTRACT OF Monodora myristiica SEED IN TYPE 2 DIABETIC WISTAR RATS

Abstract

Diabetes mellitus is a type of metabolic condition of elevated blood glucose with increase in prevalence and death rates in all geographical areas. This project examined the antioxidant and antidyslipidemic effects of oral administration of ethanolic extract of Monodora myristica seed intype 2 diabetic (T2DM) rats. Induction of T2DM was done by feeding the rats with high fat diet followed by intraperitonial administration of 30 mg streptozotocin in citrate buffer solution. Forty male wistar rats were randomized into eight groups of five rats. The first group was non-diabetic rats and received 0.5 mL distilled water, the second was diabetic and received 0.5 mL of distilled water, the third-eighth groups were diabetic rats treated with metformin, 25, 50, 100 mg/kg body weight of the extract and 10 and 20 mg of selenium nanoparticles respectively for fourteen days. Findings from the result showed increased levels of serum total cholesterol, triacylglycerides, low density lipoprotein and malondialdehyde. Administration of the extract and selenium nanoparticles brought about a decrease in these levels. The antioxidant status of the diabetic rats were reduced, however there were not significant (P>0.05) differences in the reduced glutathione (except the 10 mg selenium nanoparticles), glutathione S-transferase and peroxidase activities but a significant (P<0.05) decrease in superoxide dismutase and catalase activities when compared to the diabetic untreated rats. Conclusively, this study further corroborates the potentials of the seed in mitigating lipid-associated diabetic disorders and suggestive of its advantage as food supplement in the management of diabetes complications.