IN VITRO ANTIOXIDANT, ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE INHIBITORY EFFECT OF N-HEXANE EXTRACT OF Monodora tenuifolia SEED

Abstract

Monodora tenuifolia is a natural plant used for different purposes in Nigeria. It serves medicinal and nutrient purposes. Diabetes a global disease of fast rising incidence rate, thus, requiring effective therapeutical options. This project investigated the in vitro antioxidant potential, α-amylase and α-glucosidase inhibitory effect of n-hexane extract of Monodora tenuifolia seed. The antioxidant activity of the extract was determined using 2,2-Azino Bis (3-Ethylbenzthiazolin-6-Sulfonic Acid) (ABTS) scavenging activity, 2, 2-Diphenyl-1Picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP), total flavonoid content (TFC), total phenolic content (TPC), total antioxidant capacity (TAC) and Nitric oxide (NO) concentration. The results from this study revealed that the Monodora tenuifolia seed contained an appreciable amount of flavonoids and phenols. The Nitric oxide scavenging activity, (ABTS) scavenging activity, and DPPH radical scavenging activity were dose dependent. At the highest dose, the scavenging activity obtained for NO, ABTS, DPPH, TAC, FRAP were 55.18 ± 0.18 µg/ml, 68.07± 0.36 µg/ml, 70.57 ± 0.04 µg/ml, 6.53 ± 0.64 µg/ml and 0.32 ± 0.00µg/ml respectively. The extract also showed the ability to inhibit the activity of α-amylase and α-glucosidase. The n-hexane extract of Monodora tenuifolia demonstrated potential anti-diabetic activity probably due to the phytochemicals present