ETHANOLIC EXTRACT OF Monodora myristica SEED REGULATED HYPERGLYCEMIA AND AMELIORATED HEPATOPATHY IN TYPE 2 DIABETIC RATS

Abstract

Despite the high incidence of type 2 diabetes (T2DM) and associated complications, there has been little or no successful development of effective management options. Monodora myristica is a commonly consumed plant and this study therefore seeks to evaluate its ethanolic extract’s role in hyperglycemic regulation and hepatopathy amelioration in type 2 diabetic rats. Experimental animals were induced into T2DM by feeding the rats with high fat diet for three weeks followed by intraperitonial administration of streptozotocin. Forty male wistar rats with average weight 156.18 ± 0.05g were randomly divided into eight groups of 5 rats each and treated orally for fourteen days. Group I, non-diabetic rats were administered 0.5 mL distilled water; group II were untreated diabetic rats administered distilled water; groups III were diabetic rats administered metformin, group IV- VIII were diabetic rats administered ethanolic extract of Monodora myristica seed at 25, 100, 200 mg/kg body weight and 10mg and 20mg selenium nanoparticles of the extract respectively. The results of this study revealed that induction of T2DM caused a significant (P < 0.05) increase in the fasting blood glucose, glycosylated haemoglobin, C-Peptide, fructose-1, 6-bisphosphate and glucose-6-phosphate dehydrogenase levels of the rats. Treatment of the rats with the extract and its nanoparticles brought about a decrease in these levels while the decrease in albumin, total and direct bilirubin, insulin and adiponectin levels in diabetic rats were also reversed. Collectively, the seed demonstrated antidiabetic potential by improving insulin biosynthesis and utilization, it also mitigated the development of liver disease such fatty liver disease and as such a candidate for the management of diabetics in the diet.

Despite the high incidence of type 2 diabetes (T2DM) and associated complications, there has been little or no successful development of effective management options. Monodora myristica is a commonly consumed plant and this study therefore seeks to evaluate its ethanolic extract’s role in hyperglycemic regulation and hepatopathy amelioration in type 2 diabetic rats. Experimental animals were induced into T2DM by feeding the rats with high fat diet for three weeks followed by intraperitonial administration of streptozotocin. Forty male wistar rats with average weight 156.18 ± 0.05g were randomly divided into eight groups of 5 rats each and treated orally for fourteen days. Group I, non-diabetic rats were administered 0.5 mL distilled water; group II were untreated diabetic rats administered distilled water; groups III were diabetic rats administered metformin, group IV- VIII were diabetic rats administered ethanolic extract of Monodora myristica seed at 25, 100, 200 mg/kg body weight and 10mg and 20mg selenium nanoparticles of the extract respectively. The results of this study revealed that induction of T2DM caused a significant (P < 0.05) increase in the fasting blood glucose, glycosylated haemoglobin, C-Peptide, fructose-1, 6-bisphosphate and glucose-6-phosphate dehydrogenase levels of the rats. Treatment of the rats with the extract and its nanoparticles brought about a decrease in these levels while the decrease in albumin, total and direct bilirubin, insulin and adiponectin levels in diabetic rats were also reversed. Collectively, the seed demonstrated antidiabetic potential by improving insulin biosynthesis and utilization, it also mitigated the development of liver disease such fatty liver disease and as such a candidate for the management of diabetics in the diet.